Please contact our team on with your order number to obtain a RMA number prior to return. We also do not accept products that are intimate or sanitary goods, hazardous materials, or flammable liquids or gases. Perishable goods such as food, flowers, newspapers or magazines cannot be returned. Several types of goods are exempt from being returned. It must also be in the original packaging. To be eligible for an accepted return, your item must be new, unused and in the same condition that you received it. If 60 days have gone by since your purchase, unfortunately we can’t offer you a return, refund or exchange. SuperOffice returns policy lasts 60 days. Please be advised some countries are currently excluded in available shipping zones. Please confirm your address before purchasing. We don't take responsibility for orders that are lost or stolen once the parcel has been delivered to the correct delivery address. We utilise Australia Post International Services and International courier companies. Please “Contact Us” by emailing us at confirm accurate delivery charge to your desired country. International orders are more than welcomed. During peak periods including holiday season or promotional times, please expect slight delays with the processing of your order.Please confirm your address upon checkout. We don't take responsibility for orders that are lost or stolen once the parcel has been delivered to the correct delivery address.Should any delays occur, we will be in touch immediately. All orders are usually dispatched within 24-48 Hours of Payment on normal business days.Our express parcels are shipped via Australia Post or equivalent courier service.1-3 Business Days (depending on your location).CONTACT USĮXPRESS POST (available for selected items):įor All In Stock Items via Australia Post Express or Equivalent courier Should you have any enquiries regarding your location or postage, please do not hesitate to get in touch with us at any time.Depending on size of order and location, we may employ various reputable courier services.For bulky orders which may involve pallets, we may clarify freight costs if the destination is rural and may require forklift access.Every Parcel with Registered Postage and Tracking Information.Scale bars: 20 μm.All parcels are shipped from our distribution centre in Sydney NSW, Australia. Cells expressing mGPIHBP1-W108S did not bind cLPL-wt. ( B) Immunocytochemistry studies showing that cLPL-wt bound avidly to CHO cells expressing wild-type mGPIHBP1 (colocalization), while LPL-C420Y had no capacity to bind to cells expressing wild-type mGPIHBP1 (no colocalization). Cells expressing hGPIHBP1-W109S did not bind cLPL-wt. ( A) Immunocytochemistry studies showing that cLPL-wt bound avidly to neighboring CHO cells expressing wild-type hGPIHBP1 (hence cLPL-wt colocalized with hGPIHBP1), whereas cLPL-C420Y had no capacity to bind to cells expressing wild-type hGPIHBP1 (no colocalization). Immunocytochemistry studies were performed on permeabilized and nonpermeabilized cells with a goat antibody against the S-protein tag (red) and a mouse monoclonal antibody against the V5 tag (green). We conclude that LPL reaches the capillary lumen in chickens - as it does in mammals - despite an apparent absence of GPIHBP1.ĬHO pgsA-745 cells were transiently transfected with S-protein–tagged wild-type human (h) or mouse (m) GPIHBP1 (or hGPIHBP1-W109S or mGPIHBP1-W108S) and coplated with cells that had been transfected with V5-tagged versions of cLPL (wt or C420Y). However, we could not identify a gene for GPIHBP1 in the chicken genome, nor could we identify a transcript for GPIHBP1 in a large chicken RNA-seq data set. Remarkably, chicken LPL bound in a specific fashion to mammalian GPIHBP1. LPL was released rapidly from chicken hearts with an infusion of heparin, consistent with LPL being located inside blood vessels. When the antibodies against chicken LPL were injected intravenously into chickens, they bound to LPL on the luminal surface of capillaries in heart and adipose tissue. Using purified antibodies against chicken LPL, we showed that LPL is present on capillary endothelial cells of chicken heart and adipose tissue, colocalizing with von Willebrand factor. The obvious question is whether the LPL in lower vertebrates is able to reach the capillary lumen. In all lower vertebrate species (e.g., birds, amphibians, reptiles, fish), a gene for LPL can be found easily, but a gene for GPIHBP1 has never been found. In mammals, GPIHBP1 is absolutely essential for transporting lipoprotein lipase (LPL) to the lumen of capillaries, where it hydrolyzes the triglycerides in triglyceride-rich lipoproteins.
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